The effect of supplementing organic and inorganic trace minerals in flushing ration on hormonal profile, antioxidant status, and blood biochemical parameters of Ghezel ewes during breeding season.

چکیده مقاله

The present study was designed to compare the efficacy of organic and inorganic sources of trace minerals (TM) in the flushing diet on some blood biochemical parameters and hormonal profile of Ghezel ewes during breeding season. Eighty ewes (48.88±7.40 kg), age (2 to 4), with parity (1 to 4), and BCS (2 to 3.5) were assigned to the experiment. The study was conducted for a period of five weeks. The ewes were divided into 4 treatment groups included: 1 (CTM): flushing with chelated TM (Zn, Cu, Mn, Fe, Cr, Se and Co from Bonzaplex7), 2 (ITM): flushing with inorganic TM (Zn, Cu, Mn, Fe, Cr, Se and Co), 3 (NTM): flushing without trace minerals, and 4 (Control): only pasture grazed. At the end of five weeks, blood samples were collected on day 34 of the experiment to assay hormones, enzymes, and metabolites. The collected data were analyzed by GLM procedure of SAS software. The experimental diets had no effect on cholesterol, glucose, insulin, and estradiol concentrations (P>0.05). Progesterone tended to be higher in CTM group (P=0.07). However, the lowest triglyceride and total protein were observed in ITM group (P<0.05). Also, serum urea concentration was the highest in the control group (P<0.05). Concentrations of T3 and T4 were affected by treatments (P<0.01) and were higher in the CTM group as compared with other groups. However, the ratio of T3/T4 was higher in the NTM and control groups as compared with supplemented ewes (P<0.01). Although superoxide dismutase, alkaline phosphatase, choline-esterase, and hemoglobin were not affected by treatments (P>0.05), malondialdehyde (P=0.09) and glutathione peroxidase (P=0.07) tended to be lower in CTM group, and total antioxidant capacity was lower in CTM group (P<0.01). Therefore, the current study demonstrated that the flushing ration with TM chelate could not affect biochemical parameters, antioxidant status and sex hormones, but thyroid hormones improved in ewes. So, it is suggested to use this supplement in longer period in order to evaluate these indicators.