Autologous platelet-rich plasma promotes regeneration of ethanol-induced endometrial damage in a canine model

Abstract

Weak and damaged endometrium presents a significant challenge in managing pregnancies, often resulting in symptoms such as postpartum uterine disorders, recurrent miscarriages, and other complications. While various treatments exist, their effectiveness remains limited. Recently, scientists have turned their attention to platelet-rich plasma (PRP) for its growth factors and cytokines in human medicine. However, its potential for reconstructing endometrial issues in canine models remains unexplored. The present study aimed to investigate the therapeutic properties of PRP for thinned endometrium. For this purpose, we randomly divided 12 dogs into four groups: group E received 95% ethanol in uterine horns to create a model of endometrial damage; group E+NS received saline 72 hours post-injury; and group E+PRP received PRP 72 hours post-injury; and group NS+PRP was initially given normal saline and PRP 72 hours later. The results showed significant improvements in endometrial thickness, epithelial repair, and uterine gland density in the E+PRP group in comparison to the E group. The E+NS group did not show significant differences in comparison to the E group. The results showed a decrease in bleeding and necrosis severity in the E+PRP group. Additionally, hyperemia and hemosiderosis levels in the E+PRP, E+NS, and E groups were similar. In conclusion, the utilization of platelet-rich plasma was successful in the treatment of thinned endometrium in dogs. However, this could be a preliminary study regarding its small sample size.