| Authors | Siamak Kazemi-Darabadi - Farshid Sarrafzadeh-Rezaei - Amir-Abbas Farshid - Bahram Dalir-Naghadeh |
|---|---|
| Journal | International Journal of Surgery |
| Presented by | دانشگاه تبریز |
| Page number | 751-756 |
| Volume number | 12 |
| Paper Type | Full Paper |
| Published At | 2014 |
| Journal Grade | ISI (WOS) |
| Journal Type | Typographic |
| Journal Country | United States |
| Journal Index | Scopus |
Abstract
Background
Healing of skin wound is a multi-factorial and complex process. Treatment of diabetic wounds is still a major clinical challenge. Recently, stem cell transplantation to chronic wounds is favored. The objective of this study was to evaluate effects of pre-labeled allogenous skin fibroblasts on healing of ovine diabetic wound model.
Methods
Eight 4-month-old Iranian Makoui wethers were used in this study. Alloxan monohydrate was used for induction of diabetes. In each wether two excisional wound were created on dorsum of the animal. Wounds of one side were randomly chosen as treatment group (n = 8), and wounds of the other side were considered as control group (n = 8). Pre-labeled skin fibroblasts with bromodeoxyuridine were used in wounds of one side as treatment. Photographs were taken in distinct times for planimetric evaluation. Wound samples were taken for BrdU detection and histopathologic evaluations on day 21 post-wounding.
Results
The planimetric study showed closure of fibroblast treated wounds is significantly faster than control group (P < 0.05). Immunohistochemical staining with anti-bromodeoxyuridine antibody indicated presence of transplanted cells in the wounds. Histopathologic evaluations of H&E stained sections disclosed significantly increasing of re-epithelialization, number of fibroblasts, and number of blood vessels in treatment group in comparison to control group (P < 0.05).
Conclution
The results of this study indicated that allogenous skin fibroblast transplantation can positively affect wound healing in diabetic sheep.