شناسایی توکوپلاسما گوندی در نمونه ها ی سقط جنین گوسفندی در منطقه تبریز به روشnested pcR

چکیده مقاله

of the aborted sheep fetuses that were referred to Tabriz University Faculty of Veterinary Medicine from January 2019 to May 1400, were randomly selected. Fetuses were dissected following the health protocols and samples were prepared from different parts of the fetuses by aseptic method and transferred to sterile microtubes. The samples included liver, spleen, abomasal contents, lungs, heart and brain, which were kept at -20oC used until the time of DNA extraction. To extract DNA, cells were lysed using proteinase K, and then DNA was extracted using phenol/chloroform/isoamyl alcohol method. Quantitative and qualitative concentration measurement of the extracted DNA was performed by spectrophotometric method by Nanodrop. For PCR test, 4 primers (B1F1, B1R1, B1F2, B1R2) resulting from the B1 gene of Toxoplasma RH strain were used. The positive control sample was prepared from Tabriz University of Medical Sciences. None of the samples were infected with Toxoplasma gondii parasite, which can indicate the low percentage of this infection in East Azerbaijan province. Finally, to ensure the reference samples, a Gene Detection test was performed, and it was found that all the samples belonged to sheep.